1.
J Histochem Cytochem
; 51(9): 1237-40, 2003 Sep.
Artículo
en Inglés
| MEDLINE
| ID: mdl-12923249
RESUMEN
We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-micro m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.